8I8X

Cryo-EM Structure of OmpC3-MlaA-MlaC Complex in MSP2N2 Nanodiscs

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Outer membrane porin C	polymer	346	38336.2	3	UniProt (P06996) Pfam (PF00267)	Escherichia coli K-12	Outer membrane protein 1B,Outer membrane protein C,Porin OmpC
2	D (D)	Intermembrane phospholipid transport system lipoprotein MlaA	polymer	234	26298.3	1	UniProt (P76506) Pfam (PF04333)	Escherichia coli K-12
3	E (F)	Intermembrane phospholipid transport system binding protein MlaC	polymer	199	22903.9	1	UniProt (P0ADV7) Pfam (PF05494)	Escherichia coli K-12
4	F, G, H (A, B, C)	(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid	non-polymer		2238.7	3	Chemie (KDL)

Sequence modifications

D: 1 - 234 (UniProt: P76506)

PDB	External Database	Details
Cys 188	Gln 205	engineered mutation

F: 1 - 191 (UniProt: P0ADV7)

PDB	External Database	Details
Cys 151	Val 171	engineered mutation
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164211.0
Non-Polymers*	Number of molecules	3
	Total formula weight	6716.2
All*	Total formula weight	170927.1

*Water molecules are not included.