8I79

Cryo-EM structure of KCTD7 in complex with Cullin3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F, A, D, G, I	BTB/POZ domain-containing protein KCTD7	polymer	297	34146.6	5	UniProt (Q8BJK1) Pfam (PF02214) In PDB	Mus musculus (house mouse)
2	B, C, E, H, J	Cullin-3	polymer	376	44146.4	5	UniProt (Q13618) Pfam (PF00888) In PDB	Homo sapiens (human)	CUL-3

Sequence modifications

F, A, D, G, I: 1 - 289 (UniProt: Q8BJK1)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Tyr 126	His 126	engineered mutation

B, C, E, H, J: 22 - 388 (UniProt: Q13618)

PDB	External Database	Details
Met 13	-	initiating methionine
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Arg 342	Ile 342	engineered mutation
Asp 346	Leu 346	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	391465.3
All*	Total formula weight	391465.3

*Water molecules are not included.