8I4X

Cryo-EM structure of 5-subunit Smc5/6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Structural maintenance of chromosomes protein 5	polymer	1069	123390.0	1	UniProt (Q08204) Pfam (PF02463) In PDB	Saccharomyces cerevisiae S288C
2	B	Structural maintenance of chromosomes protein 6	polymer	1104	126889.4	1	UniProt (Q12749) Pfam (PF13476) In PDB	Saccharomyces cerevisiae S288C	DNA repair protein RHC18,Rad18 homolog
3	C	E3 SUMO-protein ligase MMS21	polymer	267	30388.3	1	UniProt (P38632) Pfam (PF22326) Pfam (PF11789) In PDB	Saccharomyces cerevisiae S288C	E3 SUMO-protein transferase MMS21,Methyl methanesulfonate-sensitivity protein 21,Non-structural maintenance of chromosome element 2,Non-SMC element 2
4	D	Nse6	polymer	378	44498.2	1	UniProt (P40026) Pfam (PF08691) In PDB	Saccharomyces cerevisiae S288C
5	E	Non-structural maintenance of chromosome element 5	polymer	556	64079.6	1	UniProt (Q03718) Pfam (PF08691) In PDB	Saccharomyces cerevisiae S288C	Non-SMC element 5

Sequence modifications

A: 25 - 1093 (UniProt: Q08204)

PDB	External Database	Details
Ala 824	Met 824	engineered mutation

B: 1 - 1104 (UniProt: Q12749)

PDB	External Database	Details
Ala 464	Leu 464	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	389245.6
All*	Total formula weight	389245.6

*Water molecules are not included.