8I3O

crystal structure of yeast cytosine deaminase mutant yCD-RQ-1/8SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Cytosine deaminase	polymer	185	20955.7	4	UniProt (Q12178) Pfam (PF00383)	Saccharomyces cerevisiae (baker's yeast)	yCD,Cytosine aminohydrolase,Fluorocytosine resistance protein 1
2	E, F, G, H (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	I, J, K, L (A, B, C, D)	water	water		18.0	509	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 158 (UniProt: Q12178)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Arg 159	-	expression tag
Ile 160	-	expression tag
Glu 161	-	expression tag
Leu 162	-	expression tag
Gln 163	-	expression tag
Lys 164	-	expression tag
Gln 165	-	expression tag
Gln 166	-	expression tag
Glu 167	-	expression tag
Glu 168	-	expression tag
Glu 169	-	expression tag
Ala 170	-	expression tag
Glu 171	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	83822.9
Non-Polymers*	Number of molecules	4
	Total formula weight	219.8
All*	Total formula weight	84042.7

*Water molecules are not included.