8HW8
Crystal structure of Heterodera glycines chitinase 2 D129A/E131A mutant in complex with nodulation factor SmNF-V (C16:2, S)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Chitinase | polymer | 356 | 39279.6 | 1 | UniProt (Q8I6X8) Pfam (PF00704) In PDB | Heterodera glycines (soybean cyst nematode) | |
2 | D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB | |||
3 | C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
4 | A | (2~{Z},9~{E})-~{N}-[(2~{R},3~{S},4~{R},5~{S},6~{S})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl]hexadeca-2,9-dienamide | non-polymer | 413.5 | 1 | Chemie (68X) | |||
5 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
6 | water | water | 18.0 | 287 | Chemie (HOH) |
Sequence modifications
A: 1 - 350 (UniProt: Q8I6X8)
PDB | External Database | Details |
---|---|---|
Ala 129 | Asp 129 | engineered mutation |
Ala 131 | Glu 131 | engineered mutation |
His 351 | - | expression tag |
His 352 | - | expression tag |
His 353 | - | expression tag |
His 354 | - | expression tag |
His 355 | - | expression tag |
His 356 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 39279.6 | |
Branched | Number of molecules | 2 |
Total formula weight | 1335.3 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 519.7 | |
All* | Total formula weight | 41134.5 |