8HW7

Crystal structure of Heterodera glycines chitinase 2 D129A/E131A mutant in complex with chitopentaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Chitinase	polymer	356	39279.6	1	UniProt (Q8I6X8) Pfam (PF00704)	Heterodera glycines (soybean cyst nematode)
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1034.0	1	In PDB GlyTouCan (G07670VS)
4	D (A)	water	water		18.0	398	Chemie (HOH)

Sequence modifications

A: 1 - 350 (UniProt: Q8I6X8)

PDB	External Database	Details
Ala 129	Asp 129	engineered mutation
Ala 131	Glu 131	engineered mutation
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39279.6
Branched	Number of molecules	2
	Total formula weight	1458.4
All*	Total formula weight	40737.9

*Water molecules are not included.