8HUI
Crystal structure of DFA I-forming Inulin Lyase from Streptomyces peucetius subsp. caesius ATCC 27952 in complex with GF4, DFA I, and fructose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Fructotransferase | polymer | 400 | 42752.5 | 3 | UniProt (A0A2D3U3Z1) | Streptomyces peucetius subsp. caesius ATCC 27952 | inulin lyase [DFA I-forming] |
| 2 | D, E (D, E) | beta-D-fructofuranose-(2-1)-alpha-D-fructofuranose | branched | 342.3 | 2 | In PDB | |||
| 3 | F, G, H, I (F, G, H, I) | beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 828.7 | 4 | In PDB | |||
| 4 | J (A) | beta-D-fructofuranose | non-polymer | 180.2 | 1 | Chemie (FRU) | |||
| 5 | K (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | L (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 7 | M (C) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 8 | N, O, P (A, B, C) | water | water | 18.0 | 943 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 394 (UniProt: A0A2D3U3Z1)
| PDB | External Database | Details |
|---|---|---|
| His 395 | - | expression tag |
| His 396 | - | expression tag |
| His 397 | - | expression tag |
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 128257.5 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 3999.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 543.6 | |
| All* | Total formula weight | 132800.6 |
