8HUI

Crystal structure of DFA I-forming Inulin Lyase from Streptomyces peucetius subsp. caesius ATCC 27952 in complex with GF4, DFA I, and fructose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Fructotransferase	polymer	400	42752.5	3	UniProt (A0A2D3U3Z1) In PDB	Streptomyces peucetius subsp. caesius ATCC 27952	inulin lyase [DFA I-forming]
2	D, E	beta-D-fructofuranose-(2-1)-alpha-D-fructofuranose	branched		342.3	2	In PDB
3	F, G, H, I	beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		828.7	4	In PDB
4	A	beta-D-fructofuranose	non-polymer		180.2	1	Chemie (FRU)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
7	C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8		water	water		18.0	943	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 394 (UniProt: A0A2D3U3Z1)

PDB	External Database	Details
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	128257.5
Branched	Number of molecules	6
	Total formula weight	3999.5
Non-Polymers*	Number of molecules	4
	Total formula weight	543.6
All*	Total formula weight	132800.6

*Water molecules are not included.