8HS3

Gi bound orphan GPR20 in ligand-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40472.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1,G protein beta subunit
3	C	Ggama	polymer	81	8722.1	1	Pfam (PF00631) UniProt (by SIFTS) (P59768) In PDB	Homo sapiens
4	R	Soluble cytochrome b562,G-protein coupled receptor 20	polymer	511	56042.0	1	UniProt (P0ABE7) UniProt (Q99678) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562
5	S	scFv16	polymer	297	31870.6	1		Homo sapiens

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

R: -106 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -132	-	initiating methionine
Lys -131	-	expression tag
Thr -130	-	expression tag
Ile -129	-	expression tag
Ile -128	-	expression tag
Ala -127	-	expression tag
Leu -126	-	expression tag
Ser -125	-	expression tag
Tyr -124	-	expression tag
Ile -123	-	expression tag
Phe -122	-	expression tag
Cys -121	-	expression tag
Leu -120	-	expression tag
Val -119	-	expression tag
Phe -118	-	expression tag
Ala -117	-	expression tag
Asp -116	-	expression tag
Tyr -115	-	expression tag
Lys -114	-	expression tag
Asp -113	-	expression tag
Asp -112	-	expression tag
Asp -111	-	expression tag
Asp -110	-	expression tag
Ala -109	-	expression tag
Gly -108	-	expression tag
Arg -107	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	-	linker

R: 1 - 358 (UniProt: Q99678)

PDB	External Database	Details
Glu 359	-	expression tag
Phe 360	-	expression tag
Leu 361	-	expression tag
Glu 362	-	expression tag
Val 363	-	expression tag
Leu 364	-	expression tag
Phe 365	-	expression tag
Gln 366	-	expression tag
Gly 367	-	expression tag
Pro 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	174523.8
All*	Total formula weight	174523.8

*Water molecules are not included.