8HS2

Orphan GPR20 in complex with Fab046

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	heavy chain of Fab046	polymer	247	26493.7	1		Rattus
2	B (C)	Light chain of Fab046	polymer	233	25637.7	1		Rattus
3	C (R)	Soluble cytochrome b562,G-protein coupled receptor 20	polymer	459	50631.0	1	UniProt (P0ABE7) UniProt (Q99678) Pfam (PF00001)	Escherichia coli	Cytochrome b-562

Sequence modifications

R: -98 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -132	-	initiating methionine
Lys -131	-	expression tag
Thr -130	-	expression tag
Ile -129	-	expression tag
Ile -128	-	expression tag
Ala -127	-	expression tag
Leu -126	-	expression tag
Ser -125	-	expression tag
Tyr -124	-	expression tag
Ile -123	-	expression tag
Phe -122	-	expression tag
Cys -121	-	expression tag
Leu -120	-	expression tag
Val -119	-	expression tag
Phe -118	-	expression tag
Ala -117	-	expression tag
Asp -116	-	expression tag
Tyr -115	-	expression tag
Lys -114	-	expression tag
Asp -113	-	expression tag
Asp -112	-	expression tag
Asp -111	-	expression tag
Asp -110	-	expression tag
Ala -109	-	expression tag
Gly -108	-	expression tag
Arg -107	-	expression tag
Ala -106	-	expression tag
Ala -105	-	expression tag
Asp -104	-	expression tag
Leu -103	-	expression tag
Glu -102	-	expression tag
Asp -101	-	expression tag
Asn -100	-	expression tag
Trp -99	-	expression tag
Ile -4	His 124	conflict
Leu 0	-	linker

R: 1 - 316 (UniProt: Q99678)

PDB	External Database	Details
Trp 139	Leu 139	engineered mutation
Asn 293	Asp 293	engineered mutation
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102762.4
All*	Total formula weight	102762.4

*Water molecules are not included.