8HS0
The mutant structure of DHAD V178W
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dihydroxy-acid dehydratase, chloroplastic | polymer | 574 | 61225.9 | 1 | UniProt (Q9LIR4) Pfam (PF00920) Pfam (PF24877) | Arabidopsis thaliana (thale cress) | AthDHAD,DAD |
| 2 | B (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 3 | C (A) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 1 | Chemie (FES) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (A) | water | water | 18.0 | 429 | Chemie (HOH) |
Sequence modifications
A: 2 - 574 (UniProt: Q9LIR4)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Trp 178 | Val 212 | engineered mutation |
| Ala 525 | Lys 559 | engineered mutation |
| Ala 526 | Lys 560 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 61225.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 292.2 | |
| All* | Total formula weight | 61518.1 |
