8HRT

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K/F100V/P192S) in complex with NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Glyceraldehyde-3-phosphate dehydrogenase	polymer	342	37101.5	4	UniProt (A0A8G0CHY2) Pfam (PF00044) Pfam (PF02800) UniProt (by SIFTS) (Q01651) In PDB	Corynebacterium glutamicum
2	A, B, C, D	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
3	A, B, C	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5		water	water		18.0	787	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 334 (UniProt: A0A8G0CHY2)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation
Lys 37	Thr 37	engineered mutation
Val 100	Phe 100	engineered mutation
Ser 192	Pro 192	engineered mutation
Leu 335	-	expression tag
Glu 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148406.0
Non-Polymers*	Number of molecules	9
	Total formula weight	3404.1
All*	Total formula weight	151810.0

*Water molecules are not included.