8HRT
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K/F100V/P192S) in complex with NADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glyceraldehyde-3-phosphate dehydrogenase | polymer | 342 | 37101.5 | 4 | UniProt (A0A8G0CHY2) Pfam (PF00044) Pfam (PF02800) UniProt (by SIFTS) (Q01651) | Corynebacterium glutamicum | |
| 2 | E, H, K, M (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
| 3 | F, G, J, L (A, B, C) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | I (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 5 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 787 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 334 (UniProt: A0A8G0CHY2)
| PDB | External Database | Details |
|---|---|---|
| Ser 36 | Leu 36 | engineered mutation |
| Lys 37 | Thr 37 | engineered mutation |
| Val 100 | Phe 100 | engineered mutation |
| Ser 192 | Pro 192 | engineered mutation |
| Leu 335 | - | expression tag |
| Glu 336 | - | expression tag |
| His 337 | - | expression tag |
| His 338 | - | expression tag |
| His 339 | - | expression tag |
| His 340 | - | expression tag |
| His 341 | - | expression tag |
| His 342 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 148406.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 3404.1 | |
| All* | Total formula weight | 151810.0 |
