8HRQ

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K) in complex with NAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	342	37159.6	2	UniProt (Q01651) Pfam (PF00044) Pfam (PF02800)	Corynebacterium glutamicum ATCC 13032	GAPDH,NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
2	C, I (A, B)	CESIUM ION	non-polymer		132.9	2	Chemie (CS)
3	D, J (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	E, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	G, K (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	H, L (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	M, N (A, B)	water	water		18.0	241	Chemie (HOH)

Sequence modifications

A, B: 1 - 334 (UniProt: Q01651)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation
Lys 37	Thr 37	engineered mutation
Leu 335	-	expression tag
Glu 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74319.1
Non-Polymers*	Number of molecules	10
	Total formula weight	2090.9
All*	Total formula weight	76410.1

*Water molecules are not included.