8HQN

Activation mechanism of GPR132 by 9(S)-HODE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (H)	scFv16	polymer	250	26610.6	1		Mus musculus
4	D (R)	Soluble cytochrome b562,Probable G-protein coupled receptor 132	polymer	510	57125.3	1	UniProt (P0ABE7) UniProt (Q9UNW8) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,G2 accumulation protein
5	E (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
6	F (R)	(9S,10E,12Z)-9-hydroxyoctadeca-10,12-dienoic acid	non-polymer		296.4	1	Chemie (9HO)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: -105 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -129	-	initiating methionine
Lys -128	-	expression tag
Thr -127	-	expression tag
Ile -126	-	expression tag
Ile -125	-	expression tag
Ala -124	-	expression tag
Leu -123	-	expression tag
Ser -122	-	expression tag
Tyr -121	-	expression tag
Ile -120	-	expression tag
Phe -119	-	expression tag
Cys -118	-	expression tag
Leu -117	-	expression tag
Val -116	-	expression tag
Phe -115	-	expression tag
Ala -114	-	expression tag
Asp -113	-	expression tag
Tyr -112	-	expression tag
Lys -111	-	expression tag
Asp -110	-	expression tag
Asp -109	-	expression tag
Asp -108	-	expression tag
Asp -107	-	expression tag
Lys -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	Arg 128	conflict

R: 1 - 380 (UniProt: Q9UNW8)
A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	170045.5
Non-Polymers*	Number of molecules	1
	Total formula weight	296.4
All*	Total formula weight	170341.9

*Water molecules are not included.