8HPA

Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in a DNA binding form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase	polymer	1029	120041.2	1	UniProt (Q5IXW8) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) UniProt (by SIFTS) (A0A7H0DN44)	Monkeypox virus
2	B (C)	DNA polymerase processivity factor component A20	polymer	426	49203.9	1	UniProt (Q5IXP2) Pfam (PF05941) UniProt (by SIFTS) (A0A7H0DNC0)	Monkeypox virus
3	C (B)	E4R	polymer	241	27883.7	1	UniProt (Q5IXS4) UniProt (by SIFTS) (M1LL92)	Monkeypox virus
4	D (E)	DNA (5'-D(P*AP*TP*GP*GP*TP*AP*GP*GP*GP*GP*AP*AP*GP*GP*AP*TP*CP*G)-3')	polymer	26	8148.3	1		DNA molecule
5	E (D)	DNA (5'-D(*CP*GP*AP*TP*CP*CP*TP*TP*CP*CP*CP*CP*TP*AP*C)-3')	polymer	23	6921.5	1		DNA molecule

Sequence modifications

A: 1 - 1006 (UniProt: Q5IXW8)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Phe 108	Leu 108	engineered mutation
Leu 411	Trp 411	engineered mutation

B: 1 - 218 (UniProt: Q5IXS4)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	212198.5
All*	Total formula weight	212198.5

*Water molecules are not included.