8HP7
Crystal structure of (S)-2-haloacid dehalogenase K152A mutant trapped with (2R)-4-amino-2-hydroxybutanoic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | (S)-2-haloacid dehalogenase | polymer | 289 | 32901.7 | 3 | UniProt (A0A0S6WSA3) | Novosphingobium sp. MBES04 | |
| 2 | D, E, F (A, B, C) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 3 | Chemie (ABU) | |||
| 3 | G, H (C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 4 | I, J, K (A, B, C) | water | water | 18.0 | 863 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 242 (UniProt: A0A0S6WSA3)
| PDB | External Database | Details |
|---|---|---|
| Met -33 | - | initiating methionine |
| Gly -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Ser -30 | - | expression tag |
| His -29 | - | expression tag |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Leu -20 | - | expression tag |
| Val -19 | - | expression tag |
| Pro -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| Met -13 | - | expression tag |
| Ala -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Met -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Met -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 152 | Lys 152 | engineered mutation |
| Lys 243 | - | expression tag |
| Leu 244 | - | expression tag |
| Ala 245 | - | expression tag |
| Ala 246 | - | expression tag |
| Ala 247 | - | expression tag |
| Leu 248 | - | expression tag |
| Glu 249 | - | expression tag |
| His 250 | - | expression tag |
| His 251 | - | expression tag |
| His 252 | - | expression tag |
| His 253 | - | expression tag |
| His 254 | - | expression tag |
| His 255 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98705.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 433.5 | |
| All* | Total formula weight | 99138.6 |
