8HOC

Cryo-EM structure of ligand histamine-bound Histamine H4 receptor Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562, Histamine H4 receptor fusion	polymer	549	62752.3	1	UniProt (P0ABE7) UniProt (Q9H3N8) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562,H4R,HH4R,AXOR35,G-protein coupled receptor 105,GPRv53,Pfi-013,SP9144
5	E (S)	Single-chain variable fragment scFv16	polymer	297	31870.6	1		Homo sapiens (human)
6	F (R)	2-(1~{H}-imidazol-5-yl)ethyl carbamimidothioate	non-polymer		170.2	1	Chemie (ITF)

Sequence modifications

R: -114 - -10 (UniProt: P0ABE7)

PDB	External Database	Details
Met -158	-	initiating methionine
Lys -157	-	expression tag
Thr -156	-	expression tag
Ile -155	-	expression tag
Ile -154	-	expression tag
Ala -153	-	expression tag
Leu -152	-	expression tag
Ser -151	-	expression tag
Tyr -150	-	expression tag
Ile -149	-	expression tag
Phe -148	-	expression tag
Cys -147	-	expression tag
Leu -146	-	expression tag
Val -145	-	expression tag
Phe -144	-	expression tag
Ala -143	-	expression tag
His -142	-	expression tag
His -141	-	expression tag
His -140	-	expression tag
His -139	-	expression tag
His -138	-	expression tag
His -137	-	expression tag
His -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
Asp -132	-	expression tag
Tyr -131	-	expression tag
Lys -130	-	expression tag
Asp -129	-	expression tag
Asp -128	-	expression tag
Asp -127	-	expression tag
Asp -126	-	expression tag
Lys -125	-	expression tag
Glu -124	-	expression tag
Asn -123	-	expression tag
Leu -122	-	expression tag
Tyr -121	-	expression tag
Phe -120	-	expression tag
Gln -119	-	expression tag
Ser -118	-	expression tag
Gly -117	-	expression tag
Ala -116	-	expression tag
Pro -115	-	expression tag
Trp -108	Met 29	conflict
Ile -13	His 124	conflict
Leu -9	-	linker
Glu -8	-	linker
Phe -7	-	linker
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker
Ser 2	-	linker
Ala 3	-	linker

R: 4 - 390 (UniProt: Q9H3N8)

PDB	External Database	Details
Ile 16	Val 16	conflict
Leu 21	Phe 21	conflict
Leu 25	Val 25	conflict
Val 35	Leu 35	conflict
Asn 51	Ser 51	conflict
Gln 114	Leu 114	conflict
Ile 129	Val 129	conflict
Leu 184	Val 184	conflict
Val 185	Ile 185	conflict
Tyr 196	Asn 196	conflict
Gly 205	Asp 205	conflict
Asn 206	His 206	conflict
Ser 218	Ala 218	conflict
Ser 222	Asn 222	conflict
Phe 233	Ser 233	conflict
Thr 236	Arg 236	conflict
Pro 239	Ser 239	conflict
Lys 242	Thr 242	conflict
Ala 245	Pro 245	conflict
Leu 260	Met 260	conflict
Leu 263	Ser 263	conflict
Gln 266	Lys 266	conflict
Ile 271	Thr 271	conflict
Leu 279	Phe 279	conflict
Leu 285	Val 285	conflict
Leu 293	Val 293	conflict
Lys 300	Arg 300	conflict
Pro 330	Ser 330	conflict
Arg 334	Gly 334	conflict
Phe 356	Leu 356	conflict
Ile 380	Leu 380	conflict
Asn 385	Ser 385	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	180316.0
Non-Polymers*	Number of molecules	1
	Total formula weight	170.2
All*	Total formula weight	180486.2

*Water molecules are not included.