8HOB

The cryo-EM structure of cellobiose phosphorylase from Clostridium thermocellum ( variant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cellobiose phosphorylase	polymer	820	93973.3	2	UniProt (Q8VP44) Pfam (PF06165) Pfam (PF17167) Pfam (PF03633) In PDB	Acetivibrio thermocellus
2		water	water		18.0	333	Chemie (HOH)

Sequence modifications

A, B: 3 - 812 (UniProt: Q8VP44)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 46	Cys 45	engineered mutation
Ser 98	Cys 97	engineered mutation
Ser 154	Cys 153	engineered mutation
Ser 337	Cys 336	engineered mutation
Ser 530	Cys 529	engineered mutation
Ser 592	Cys 591	engineered mutation
Ser 653	Cys 652	engineered mutation
Ser 661	Cys 660	engineered mutation
Ser 748	Cys 747	engineered mutation
Leu 813	-	expression tag
Glu 814	-	expression tag
His 815	-	expression tag
His 816	-	expression tag
His 817	-	expression tag
His 818	-	expression tag
His 819	-	expression tag
His 820	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	187946.5
All*	Total formula weight	187946.5

*Water molecules are not included.