8HO9

The cryo-EM structure of cellobiose phosphorylase from Clostridium thermocellum (cysteine-to-serine varient)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cellobiose phosphorylase	polymer	820	93941.1	2	UniProt (Q8VP44)	Acetivibrio thermocellus
2	C, D (A, B)	water	water		18.0	347	Chemie (HOH)

Sequence modifications

A, B: 3 - 812 (UniProt: Q8VP44)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 46	Cys 45	engineered mutation
Ser 98	Cys 97	engineered mutation
Ser 154	Cys 153	engineered mutation
Ser 337	Cys 336	engineered mutation
Ser 485	Cys 484	engineered mutation
Ser 490	Cys 489	engineered mutation
Ser 530	Cys 529	engineered mutation
Ser 592	Cys 591	engineered mutation
Ser 653	Cys 652	engineered mutation
Ser 661	Cys 660	engineered mutation
Ser 748	Cys 747	engineered mutation
Leu 813	-	expression tag
Glu 814	-	expression tag
His 815	-	expression tag
His 816	-	expression tag
His 817	-	expression tag
His 818	-	expression tag
His 819	-	expression tag
His 820	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	187882.3
All*	Total formula weight	187882.3

*Water molecules are not included.