8HIX
Cryo-EM structure of GPR21_m5_Gs
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 249 | 28964.7 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1,G protein beta subunit |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 96 | 10366.8 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nb35 | polymer | 149 | 16054.2 | 1 | Homo sapiens | ||
| 5 | E (R) | Probable G-protein coupled receptor 21 | polymer | 336 | 38360.7 | 1 | UniProt (Q99679) Pfam (PF00001) | Homo sapiens (human) |
Sequence modifications
A: 5 - 195 (UniProt: P63092)
A: 204 - 394 (UniProt: P63092)
G: 1 - 71 (UniProt: P59768)
R: 1 - 327 (UniProt: Q99679)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 196 | - | linker |
| Gly 197 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 249 | engineered mutation |
| Asp 252 | Ser 252 | engineered mutation |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Ala 372 | Ile 372 | engineered mutation |
| Ile 375 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Gly -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Ile -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Ser -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 68 | Cys 68 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Trp 118 | Ala 118 | conflict |
| Pro 301 | Cys 301 | conflict |
| Ala 305 | Ser 305 | conflict |
| Asp 308 | Asn 308 | conflict |
| Thr 310 | Val 310 | conflict |
| Glu 328 | - | expression tag |
| Phe 329 | - | expression tag |
| Leu 330 | - | expression tag |
| Glu 331 | - | expression tag |
| Val 332 | - | expression tag |
| Leu 333 | - | expression tag |
| Phe 334 | - | expression tag |
| Gln 335 | - | expression tag |
| Gly 336 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 131163.3 | |
| All* | Total formula weight | 131163.3 |
