8HIL

A cryo-EM structure of B. oleracea RNA polymerase V at 3.57 Angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase V largest subunit	polymer	2032	222800.8	1		Brassica oleracea
2	B	DNA-dependent RNA polymerase IV and V subunit 2	polymer	1169	132377.8	1		Brassica oleracea
3	C	RPOLD domain-containing protein	polymer	319	35484.1	1	UniProt (A0A0D3D418) Pfam (PF01193) Pfam (PF01000) In PDB	Brassica oleracea (wild cabbage)
4	E	DNA-directed RNA polymerases I, II, and III subunit RPABC1	polymer	230	26260.1	1	UniProt (A0A0D3DTU3) Pfam (PF03871) Pfam (PF01191) In PDB	Brassica oleracea (wild cabbage)
5	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	144	16686.3	1	UniProt (A0A0D3BZZ8) Pfam (PF01192) In PDB	Brassica oleracea (wild cabbage)
6	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	146	16607.3	1	UniProt (A0A0D3DUD8) Pfam (PF03870) In PDB	Brassica oleracea (wild cabbage)
7	I	DNA-directed RNA polymerase subunit	polymer	114	13123.8	1	UniProt (A0A3P6GD79) Pfam (PF02150) Pfam (PF01096) In PDB	Brassica oleracea (wild cabbage)
8	J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	71	8167.5	1	UniProt (A0A0D3AAT3) Pfam (PF01194) In PDB	Brassica oleracea (wild cabbage)
9	K	RNA_pol_L_2 domain-containing protein	polymer	116	13538.3	1	UniProt (A0A0D3DS91) Pfam (PF13656) In PDB	Brassica oleracea (wild cabbage)
10	L	DNA-directed RNA polymerases II, IV and V subunit 12	polymer	51	5983.8	1	UniProt (A0A0D2ZPP3) Pfam (PF03604) In PDB	Brassica oleracea (wild cabbage)
11	A, C, I, J, L	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
12	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

C: 1 - 319 (UniProt: A0A0D3D418)

PDB	External Database	Details
Thr 3	Ser 3	variant

E: 1 - 230 (UniProt: A0A0D3DTU3)

PDB	External Database	Details
Gly 101	Ser 101	variant
Gln 182	His 182	variant
Ile 210	Val 210	variant

F: 1 - 144 (UniProt: A0A0D3BZZ8)

PDB	External Database	Details
Glu 51	Asp 51	variant

L: 1 - 51 (UniProt: A0A0D2ZPP3)

PDB	External Database	Details
Glu 18	Lys 18	variant
Cys 32	Arg 32	variant

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	491029.7
Non-Polymers*	Number of molecules	7
	Total formula weight	416.8
All*	Total formula weight	491446.5

*Water molecules are not included.