8HG1
The structure of MPXV polymerase holoenzyme in replicating state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 1031 | 119939.1 | 1 | UniProt (A0A2L0AR76) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) UniProt (by SIFTS) (A0A7H0DN44) | Monkeypox virus (monkeypox) | |
| 2 | B (B) | E4R | polymer | 218 | 25107.7 | 1 | UniProt (Q5IXS4) UniProt (by SIFTS) (M1LL92) | Monkeypox virus (monkeypox) | MPXV-COP-095,MPXV-SL-095,MPXV-WRAIR095,MPXVgp101,Uracil-DNA glycosylase,Uracil-DNA glycosylase DNA polymerase,Uracil-DNA glycosylase interacts with A20R,DNA polymerase processivity factor |
| 3 | C (C) | DNA polymerase processivity factor component A20 | polymer | 426 | 49203.9 | 1 | UniProt (Q5IXP2) Pfam (PF05941) UniProt (by SIFTS) (A0A7H0DNC0) | Monkeypox virus (monkeypox) | |
| 4 | D (P) | DNA (25-MER) | polymer | 25 | 7682.0 | 1 | Monkeypox virus | ||
| 5 | E (T) | DNA (38-MER) | polymer | 38 | 11677.5 | 1 | Monkeypox virus | ||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) |
Sequence modifications
A: 1 - 1006 (UniProt: A0A2L0AR76)
| PDB | External Database | Details |
|---|---|---|
| Met -24 | - | initiating methionine |
| Trp -23 | - | expression tag |
| Ser -22 | - | expression tag |
| His -21 | - | expression tag |
| Pro -20 | - | expression tag |
| Gln -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Glu -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Ser -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Trp -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Phe 108 | Leu 108 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 213610.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 506.5 | |
| All* | Total formula weight | 214116.7 |
