8HCQ

Cryo-EM structure of endothelin1-bound ETAR-Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha-1	polymer	246	28084.8	1		Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	E	scFv16	polymer	285	30363.0	1		Mus musculus
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	L	Endothelin-1	polymer	21	2498.0	1	UniProt (P05305) Pfam (PF00322) In PDB	Homo sapiens (human)	Preproendothelin-1,PPET1
6	R	Endothelin-1 receptor,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera	polymer	622	69969.9	1	UniProt (P25101) UniProt (Q9GV45) Pfam (PF00001) In PDB	Homo sapiens (human)	Endothelin receptor type A,ET-A,ETA-R,hET-AR,19kOLase

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

R: 20 - 406 (UniProt: P25101)

PDB	External Database	Details
Met -57	-	initiating methionine
Asp -56	-	expression tag
Ser -55	-	expression tag
Lys -54	-	expression tag
Gly -53	-	expression tag
Ser -52	-	expression tag
Ser -51	-	expression tag
Gln -50	-	expression tag
Lys -49	-	expression tag
Gly -48	-	expression tag
Ser -47	-	expression tag
Arg -46	-	expression tag
Leu -45	-	expression tag
Leu -44	-	expression tag
Leu -43	-	expression tag
Leu -42	-	expression tag
Leu -41	-	expression tag
Val -40	-	expression tag
Val -39	-	expression tag
Ser -38	-	expression tag
Asn -37	-	expression tag
Leu -36	-	expression tag
Leu -35	-	expression tag
Leu -34	-	expression tag
Cys -33	-	expression tag
Gln -32	-	expression tag
Gly -31	-	expression tag
Val -30	-	expression tag
Val -29	-	expression tag
Ser -28	-	expression tag
Asp -27	-	expression tag
Tyr -26	-	expression tag
Lys -25	-	expression tag
Asp -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Val -20	-	expression tag
Asp -19	-	expression tag
Met -18	-	expression tag
Gly -17	-	expression tag
Gln -16	-	expression tag
Pro -15	-	expression tag
Gly -14	-	expression tag
Asn -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Phe -9	-	expression tag
Leu -8	-	expression tag
Leu -7	-	expression tag
Ala -6	-	expression tag
Pro -5	-	expression tag
Asn -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Asp 2	-	expression tag
His 3	-	expression tag
Asp 4	-	expression tag
Val 5	-	expression tag
Thr 6	-	expression tag
Gln 7	-	expression tag
Gln 8	-	expression tag
Arg 9	-	expression tag
Asp 10	-	expression tag
Glu 11	-	expression tag
Glu 12	-	expression tag
Asn 13	-	expression tag
Leu 14	-	expression tag
Tyr 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Ala 19	-	expression tag

R: 407 - 564 (UniProt: Q9GV45)

PDB	External Database	Details
Val 407	Thr 27	conflict
Glu 411	Ala 31	conflict
Glu 418	Gln 38	conflict
Ala 422	Gly 42	conflict
Leu 425	Gln 45	conflict
Val 434	Leu 54	conflict
Leu 438	Phe 58	conflict
Asn 440	Ala 60	conflict
Ala 442	Gly 62	conflict
Arg 450	Lys 70	conflict
Ile 451	Val 71	conflict
Arg 453	Leu 73	conflict
Ala 458	Gly 78	conflict
Ile 461	Ala 81	conflict
Ala 474	Gly 94	conflict
Asp 475	Phe 95	conflict
Ala 478	Gly 98	conflict
Gln 479	Leu 99	conflict
Glu 482	Met 102	conflict
Val 483	Ile 103	conflict
Val 497	Ile 117	conflict
Pro 500	His 120	conflict
Leu 514	Ile 134	conflict
Asn 515	Asp 135	conflict
Glu 522	Pro 142	conflict
Lys 531	Gln 151	conflict
Ile 545	Tyr 165	conflict
Thr 551	Asn 171	conflict
Met 556	Leu 176	conflict
Ser 564	Gly 184	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	179832.7
All*	Total formula weight	179832.7

*Water molecules are not included.