8HBD

Cryo-EM structure of IRL1620-bound ETBR-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (H)	scFv16	polymer	285	30363.0	1		Mus musculus
5	E (L)	IRL1620	polymer	15	1837.0	1		Homo sapiens
6	F (R)	Endothelin receptor type B,Endothelin receptor type B,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera	polymer	603	67882.3	1	UniProt (P24530) Pfam (PF00001)	Homo sapiens (human)	ET-B,ET-BR,Endothelin receptor non-selective type

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 27 - 424 (UniProt: P24530)

PDB	External Database	Details
Met -20	-	initiating methionine
Asp -19	-	expression tag
Ser -18	-	expression tag
Lys -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gln -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Arg -9	-	expression tag
Leu -8	-	expression tag
Leu -7	-	expression tag
Leu -6	-	expression tag
Leu -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Val -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Leu 2	-	expression tag
Leu 3	-	expression tag
Cys 4	-	expression tag
Gln 5	-	expression tag
Gly 6	-	expression tag
Val 7	-	expression tag
Val 8	-	expression tag
Ser 9	-	expression tag
Asp 10	-	expression tag
Tyr 11	-	expression tag
Lys 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Asp 16	-	expression tag
Val 17	-	expression tag
Asp 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag

R: 425 - 582 (PDB: 8HBD)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	189444.4
All*	Total formula weight	189444.4

*Water molecules are not included.