8H99
Crystal structure of E. coli ThrS catalytic domain mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Threonine--tRNA ligase | polymer | 410 | 47911.5 | 2 | UniProt (E2QMS9) Pfam (PF00587) Pfam (PF03129) UniProt (by SIFTS) (P0A8M3) In PDB | Escherichia coli | Threonyl-tRNA synthetase,ThrRS |
2 | A, B | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
3 | A, B | IMIDAZOLE | non-polymer | 69.1 | 2 | Chemie (IMD) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
5 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
6 | water | water | 18.0 | 698 | Chemie (HOH) |
Sequence modifications
A, B: 242 - 642 (UniProt: E2QMS9)
PDB | External Database | Details |
---|---|---|
Met 241 | - | initiating methionine |
Phe 462 | Tyr 462 | engineered mutation |
Leu 643 | - | expression tag |
Glu 644 | - | expression tag |
His 645 | - | expression tag |
His 646 | - | expression tag |
His 647 | - | expression tag |
His 648 | - | expression tag |
His 649 | - | expression tag |
His 650 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 95823.0 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1380.6 | |
All* | Total formula weight | 97203.6 |