8H7G
Cryo-EM structure of the human SAGA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Transformation/transcription domain-associated protein | polymer | 3859 | 438139.5 | 1 | UniProt (Q9Y4A5) Pfam (PF20175) Pfam (PF20206) Pfam (PF02259) Pfam (PF00454) | Homo sapiens (human) | 350/400 kDa PCAF-associated factor,PAF350/400,STAF40,Tra1 homolog |
| 2 | B (A) | Splicing factor 3B subunit 3 | polymer | 1217 | 135718.8 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 3 | C (B) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) | Homo sapiens (human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 4 | D (D) | Transcription factor SPT20 homolog | polymer | 779 | 85884.9 | 1 | UniProt (Q8NEM7) Pfam (PF12090) | Homo sapiens (human) | p38-interacting protein,p38IP |
| 5 | E (E) | Transcription initiation protein SPT3 homolog | polymer | 317 | 35840.8 | 1 | UniProt (O75486) Pfam (PF02269) | Homo sapiens (human) | SPT3-like protein |
| 6 | F (G) | Transcriptional adapter 1 | polymer | 374 | 41797.0 | 1 | UniProt (Q96BN2) Pfam (PF12767) | Homo sapiens (human) | SPT3-associated factor 42,STAF42,Transcriptional adapter 1-like protein |
| 7 | G (H) | TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L | polymer | 589 | 66223.0 | 1 | UniProt (O75529) Pfam (PF04494) Pfam (PF00400) | Homo sapiens (human) | TAF5L,PCAF-associated factor 65 beta,PAF65-beta |
| 8 | H (I) | STAGA complex 65 subunit gamma | polymer | 455 | 50705.3 | 1 | UniProt (O94864) Pfam (PF07524) | Homo sapiens (human) | Adenocarcinoma antigen ART1,SPTF-associated factor 65 gamma,STAF65gamma,Suppressor of Ty 7-like |
| 9 | I (K) | TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L | polymer | 622 | 67903.3 | 1 | UniProt (Q9Y6J9) Pfam (PF02969) Pfam (PF07571) | Homo sapiens (human) | TAF6L,PCAF-associated factor 65-alpha,PAF65-alpha |
| 10 | J (M) | Transcription initiation factor TFIID subunit 9 | polymer | 264 | 29006.8 | 1 | UniProt (Q16594) Pfam (PF02291) | Homo sapiens (human) | RNA polymerase II TBP-associated factor subunit G,STAF31/32,Transcription initiation factor TFIID 31 kDa subunit,TAFII-31,TAFII31,Transcription initiation factor TFIID 32 kDa subunit,TAFII-32,TAFII32 |
| 11 | K (O) | Transcription initiation factor TFIID subunit 10 | polymer | 218 | 21731.2 | 1 | UniProt (Q12962) Pfam (PF03540) | Homo sapiens (human) | STAF28,Transcription initiation factor TFIID 30 kDa subunit,TAF(II)30,TAFII-30,TAFII30 |
| 12 | L (R) | Transcription initiation factor TFIID subunit 12 | polymer | 161 | 17948.5 | 1 | UniProt (Q16514) Pfam (PF03847) | Homo sapiens (human) | Transcription initiation factor TFIID 20/15 kDa subunits,TAFII-20/TAFII-15,TAFII20/TAFII15 |
| 13 | M (X) | Unassigned sequence | polymer | 19 | 1635.0 | 1 | Homo sapiens (human) | ||
| 14 | N (L) | Ataxin-7 | polymer | 892 | 95597.7 | 1 | UniProt (O15265) Pfam (PF08313) | Homo sapiens (human) | Spinocerebellar ataxia type 7 protein |
Sequence modifications
G: 1 - 335 (UniProt: Q96BN2)
I: 1 - 414 (UniProt: O94864)
| PDB | External Database | Details |
|---|---|---|
| Met -38 | - | initiating methionine |
| Asp -37 | - | expression tag |
| Tyr -36 | - | expression tag |
| Lys -35 | - | expression tag |
| Asp -34 | - | expression tag |
| His -33 | - | expression tag |
| Asp -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Asp -30 | - | expression tag |
| Tyr -29 | - | expression tag |
| Lys -28 | - | expression tag |
| Asp -27 | - | expression tag |
| His -26 | - | expression tag |
| Asp -25 | - | expression tag |
| Ile -24 | - | expression tag |
| Asp -23 | - | expression tag |
| Tyr -22 | - | expression tag |
| Lys -21 | - | expression tag |
| Asp -20 | - | expression tag |
| Asp -19 | - | expression tag |
| Asp -18 | - | expression tag |
| Asp -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Val -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Asp 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 415 | - | expression tag |
| Ser 416 | - | expression tag |
| Glu 417 | - | expression tag |
| Asn 418 | - | expression tag |
| Leu 419 | - | expression tag |
| Tyr 420 | - | expression tag |
| Phe 421 | - | expression tag |
| Gln 422 | - | expression tag |
| Gly 423 | - | expression tag |
| Ser 424 | - | expression tag |
| Gly 425 | - | expression tag |
| Thr 426 | - | expression tag |
| Ser 427 | - | expression tag |
| Thr 428 | - | expression tag |
| Ala 429 | - | expression tag |
| Trp 430 | - | expression tag |
| Ser 431 | - | expression tag |
| His 432 | - | expression tag |
| Pro 433 | - | expression tag |
| Gln 434 | - | expression tag |
| Phe 435 | - | expression tag |
| Glu 436 | - | expression tag |
| Lys 437 | - | expression tag |
| Thr 438 | - | expression tag |
| Gly 439 | - | expression tag |
| Val 440 | - | expression tag |
| Ser 441 | - | expression tag |
| Ile 442 | - | expression tag |
| Thr 443 | - | expression tag |
| Ser 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Ser 447 | - | expression tag |
| Trp 448 | - | expression tag |
| Ser 449 | - | expression tag |
| His 450 | - | expression tag |
| Pro 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Phe 453 | - | expression tag |
| Glu 454 | - | expression tag |
| Lys 455 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 1098281.5 | |
| All* | Total formula weight | 1098281.5 |
