8H5M
Crystal structure of PETase S121E/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 300 | 31576.1 | 1 | UniProt (A0A0K8P6T7) Pfam (PF01738) | Ideonella sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | B (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 3 | C (A) | water | water | 18.0 | 222 | Chemie (HOH) |
Sequence modifications
A: 34 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Gly 14 | - | expression tag |
| Ser 15 | - | expression tag |
| Ser 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Gly 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Val 27 | - | expression tag |
| Pro 28 | - | expression tag |
| Arg 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Ser 31 | - | expression tag |
| His 32 | - | expression tag |
| Met 33 | - | expression tag |
| Glu 121 | Ser 121 | engineered mutation |
| His 186 | Asp 186 | engineered mutation |
| Cys 233 | Asn 233 | engineered mutation |
| Thr 242 | Ser 242 | engineered mutation |
| Asp 246 | Asn 246 | engineered mutation |
| Cys 282 | Ser 282 | engineered mutation |
| Leu 291 | - | expression tag |
| Glu 292 | - | expression tag |
| Asp 293 | - | expression tag |
| Pro 294 | - | expression tag |
| Ala 295 | - | expression tag |
| Ala 296 | - | expression tag |
| Asn 297 | - | expression tag |
| Lys 298 | - | expression tag |
| Ala 299 | - | expression tag |
| Arg 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Glu 302 | - | expression tag |
| Ala 303 | - | expression tag |
| Glu 304 | - | expression tag |
| Leu 305 | - | expression tag |
| Ala 306 | - | expression tag |
| Ala 307 | - | expression tag |
| Ala 308 | - | expression tag |
| Thr 309 | - | expression tag |
| Ala 310 | - | expression tag |
| Glu 311 | - | expression tag |
| Gln 312 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31576.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 31600.4 |
