8H5L
Crystal structure of PETase N37D/S121E/R132E/A171C/A180V/P181V/D186H/S193C/A202C/V211C/S214Y/R224E/N233C/S242T/N246D/N275C/S282C/F284C mutant from Ideonella sakaiensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, E) | Poly(ethylene terephthalate) hydrolase | polymer | 300 | 31656.3 | 3 | UniProt (A0A0K8P6T7) Pfam (PF01738) | Ideonella sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | D, E, M (A, E) | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 3 | F, G, H, I, J... (A, B, E) | IODIDE ION | non-polymer | 126.9 | 8 | Chemie (IOD) | |||
| 4 | O, P, Q (A, B, E) | water | water | 18.0 | 1038 | Chemie (HOH) |
Sequence modifications
A, B, E: 34 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Gly 14 | - | expression tag |
| Ser 15 | - | expression tag |
| Ser 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Gly 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Val 27 | - | expression tag |
| Pro 28 | - | expression tag |
| Arg 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Ser 31 | - | expression tag |
| His 32 | - | expression tag |
| Met 33 | - | expression tag |
| Asp 37 | Asn 37 | engineered mutation |
| Glu 121 | Ser 121 | engineered mutation |
| Glu 132 | Arg 132 | engineered mutation |
| Cys 171 | Ala 171 | engineered mutation |
| Val 180 | Ala 180 | engineered mutation |
| Val 181 | Pro 181 | engineered mutation |
| His 186 | Asp 186 | engineered mutation |
| Cys 193 | Ser 193 | engineered mutation |
| Cys 202 | Ala 202 | engineered mutation |
| Cys 211 | Val 211 | engineered mutation |
| Tyr 214 | Ser 214 | engineered mutation |
| Glu 224 | Arg 224 | engineered mutation |
| Cys 233 | Asn 233 | engineered mutation |
| Thr 242 | Ser 242 | engineered mutation |
| Asp 246 | Asn 246 | engineered mutation |
| Cys 275 | Asn 275 | engineered mutation |
| Cys 282 | Ser 282 | engineered mutation |
| Cys 284 | Phe 284 | engineered mutation |
| Leu 291 | - | expression tag |
| Glu 292 | - | expression tag |
| Asp 293 | - | expression tag |
| Pro 294 | - | expression tag |
| Ala 295 | - | expression tag |
| Ala 296 | - | expression tag |
| Asn 297 | - | expression tag |
| Lys 298 | - | expression tag |
| Ala 299 | - | expression tag |
| Arg 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Glu 302 | - | expression tag |
| Ala 303 | - | expression tag |
| Glu 304 | - | expression tag |
| Leu 305 | - | expression tag |
| Ala 306 | - | expression tag |
| Ala 307 | - | expression tag |
| Ala 308 | - | expression tag |
| Thr 309 | - | expression tag |
| Ala 310 | - | expression tag |
| Glu 311 | - | expression tag |
| Gln 312 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 94969.0 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1132.5 | |
| All* | Total formula weight | 96101.5 |
