8H5K

Crystal structure of PETase N37D/S121E/R132E/A171C/A180V/P181V/D186H/S193C/R224E/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Poly(ethylene terephthalate) hydrolase	polymer	260	27370.5	1	UniProt (A0A0K8P6T7) Pfam (PF01738)	Ideonella sakaiensis	PET hydrolase,PETase,PET-digesting enzyme
2	B (A)	water	water		18.0	329	Chemie (HOH)

Sequence modifications

A: 34 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Ser 31	-	expression tag
His 32	-	expression tag
Met 33	-	expression tag
Asp 37	Asn 37	engineered mutation
Glu 121	Ser 121	engineered mutation
Glu 132	Arg 132	engineered mutation
Cys 171	Ala 171	engineered mutation
Val 180	Ala 180	engineered mutation
Val 181	Pro 181	engineered mutation
His 186	Asp 186	engineered mutation
Cys 193	Ser 193	engineered mutation
Glu 224	Arg 224	engineered mutation
Cys 233	Asn 233	engineered mutation
Thr 242	Ser 242	engineered mutation
Asp 246	Asn 246	engineered mutation
Cys 282	Ser 282	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27370.5
All*	Total formula weight	27370.5

*Water molecules are not included.