8H5J
Crystal structure of PETase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Poly(ethylene terephthalate) hydrolase | polymer | 300 | 31606.2 | 2 | UniProt (A0A0K8P6T7) Pfam (PF12740) | Ideonella sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | C, F, I (A, B) | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 3 | D, E, G (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
| 4 | H (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | J, K (A, B) | water | water | 18.0 | 523 | Chemie (HOH) |
Sequence modifications
A, B: 34 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Gly 14 | - | expression tag |
| Ser 15 | - | expression tag |
| Ser 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Gly 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Val 27 | - | expression tag |
| Pro 28 | - | expression tag |
| Arg 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Ser 31 | - | expression tag |
| His 32 | - | expression tag |
| Met 33 | - | expression tag |
| Glu 121 | Ser 121 | engineered mutation |
| Val 180 | Ala 180 | engineered mutation |
| Val 181 | Pro 181 | engineered mutation |
| His 186 | Asp 186 | engineered mutation |
| Cys 233 | Asn 233 | engineered mutation |
| Thr 242 | Ser 242 | engineered mutation |
| Asp 246 | Asn 246 | engineered mutation |
| Cys 282 | Ser 282 | engineered mutation |
| Leu 291 | - | expression tag |
| Glu 292 | - | expression tag |
| Asp 293 | - | expression tag |
| Pro 294 | - | expression tag |
| Ala 295 | - | expression tag |
| Ala 296 | - | expression tag |
| Asn 297 | - | expression tag |
| Lys 298 | - | expression tag |
| Ala 299 | - | expression tag |
| Arg 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Glu 302 | - | expression tag |
| Ala 303 | - | expression tag |
| Glu 304 | - | expression tag |
| Leu 305 | - | expression tag |
| Ala 306 | - | expression tag |
| Ala 307 | - | expression tag |
| Ala 308 | - | expression tag |
| Thr 309 | - | expression tag |
| Ala 310 | - | expression tag |
| Glu 311 | - | expression tag |
| Gln 312 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 63212.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 494.3 | |
| All* | Total formula weight | 63706.7 |
