8H48

Blasnase-T13A/P55F with L-asn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	L-asparaginase	polymer	328	36290.1	2	UniProt (A0A6I7U6Y2) Pfam (PF00710) Pfam (PF17763)	Bacillus paralicheniformis
2	C, D, E, F, L... (A, B)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
3	G, H, I, J, N... (A, B)	FORMIC ACID	non-polymer		46.0	10	Chemie (FMT)
4	K, T (A, B)	ASPARAGINE	non-polymer		132.1	2	Chemie (ASN)
5	U, V (A, B)	water	water		18.0	432	Chemie (HOH)

Sequence modifications

A, B: 1 - 322 (UniProt: A0A6I7U6Y2)

PDB	External Database	Details
Ala 13	Thr 13	engineered mutation
Phe 55	Pro 55	engineered mutation
Leu 323	-	expression tag
Glu 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72580.2
Non-Polymers*	Number of molecules	18
	Total formula weight	870.3
All*	Total formula weight	73450.5

*Water molecules are not included.