8H3F

Cryo-EM Structure of the KBTBD2-CRL3-CSN complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	COP9 signalosome complex subunit 5	polymer	334	37621.7	1	UniProt (Q92905) Pfam (PF01398) Pfam (PF18323)	Homo sapiens (human)
2	B (A)	COP9 signalosome complex subunit 1	polymer	527	59122.2	1	UniProt (Q13098) Pfam (PF10602) Pfam (PF01399) Pfam (PF21151)	Homo sapiens (human)	SGN1,Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH
3	C (B)	COP9 signalosome complex subunit 2	polymer	443	51664.6	1	UniProt (P61201) Pfam (PF01399)	Homo sapiens (human)	SGN2,Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TR-interacting protein 15,TRIP-15
4	D (C)	COP9 signalosome complex subunit 3	polymer	441	49994.3	1	UniProt (Q9UNS2) Pfam (PF22788) Pfam (PF01399) Pfam (PF21215)	Homo sapiens (human)	SGN3,Signalosome subunit 3,JAB1-containing signalosome subunit 3
5	E (D)	COP9 signalosome complex subunit 4	polymer	406	46322.7	1	UniProt (Q9BT78) Pfam (PF18420) Pfam (PF22241) Pfam (PF01399)	Homo sapiens (human)	SGN4,Signalosome subunit 4,JAB1-containing signalosome subunit 4
6	F (F)	COP9 signalosome complex subunit 6	polymer	327	36203.4	1	UniProt (Q7L5N1) Pfam (PF01398) Pfam (PF13012)	Homo sapiens (human)	SGN6,Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP
7	G (G)	COP9 signalosome complex subunit 7b	polymer	220	24758.4	1	UniProt (Q9H9Q2) Pfam (PF22061) Pfam (PF01399) Pfam (PF18392)	Homo sapiens (human)	SGN7b,Signalosome subunit 7b,JAB1-containing signalosome subunit 7b
8	H (H)	COP9 signalosome complex subunit 8	polymer	209	23245.5	1	UniProt (Q99627) Pfam (PF10075)	Homo sapiens (human)	SGN8,Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8
9	I, K (I, M)	Kelch repeat and BTB domain-containing protein 2	polymer	636	73112.2	2	UniProt (Q8IY47) Pfam (PF00651) Pfam (PF07707) Pfam (PF01344)	Homo sapiens (human)	BTB and kelch domain-containing protein 1
10	J (R)	E3 ubiquitin-protein ligase RBX1	polymer	121	13970.8	1	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
11	L (L)	Cullin-3	polymer	768	89063.3	1	UniProt (Q13618) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (human)	CUL-3
12	M, N, O, P (E, R)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 52 - 527 (UniProt: Q13098)

PDB	External Database	Details
Met 1	-	initiating methionine
Arg 2	-	expression tag
Asp 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
Ala 6	-	expression tag
Pro 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Ala 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Ser 13	-	expression tag
Val 14	-	expression tag
Thr 15	-	expression tag
Asp 16	-	expression tag
Leu 17	-	expression tag
Tyr 18	-	expression tag
Cys 19	-	expression tag
Thr 20	-	expression tag
Pro 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
Arg 25	-	expression tag
Ser 26	-	expression tag
Asp 27	-	expression tag
Leu 28	-	expression tag
Val 29	-	expression tag
Leu 30	-	expression tag
Pro 31	-	expression tag
Gly 32	-	expression tag
Thr 33	-	expression tag
Ala 34	-	expression tag
Gly 35	-	expression tag
Asp 36	-	expression tag
Phe 37	-	expression tag
Ser 38	-	expression tag
Leu 39	-	expression tag
Ser 40	-	expression tag
Ala 41	-	expression tag
Ser 42	-	expression tag
Leu 43	-	expression tag
Ser 44	-	expression tag
Ala 45	-	expression tag
Cys 46	-	expression tag
Thr 47	-	expression tag
Leu 48	-	expression tag
Leu 49	-	expression tag
Tyr 50	-	expression tag
Glu 51	-	expression tag
-	Arg 103	deletion
-	Ser 104	deletion
-	Ser 105	deletion
-	Leu 106	deletion

C: 1 - 423 (UniProt: Q9UNS2)

PDB	External Database	Details
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

I, M: 1 - 623 (UniProt: Q8IY47)

PDB	External Database	Details
Asp 252	Ser 252	engineered mutation
Glu 624	-	expression tag
Asn 625	-	expression tag
Leu 626	-	expression tag
Tyr 627	-	expression tag
Phe 628	-	expression tag
Gln 629	-	expression tag
Gly 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag
His 634	-	expression tag
His 635	-	expression tag
His 636	-	expression tag

R: 1 - 108 (UniProt: P62877)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	578191.2
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	578452.8

*Water molecules are not included.