8H3A
Cryo-EM Structure of the KBTBD2-CRL3~N8(removed)-CSN complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | COP9 signalosome complex subunit 5 | polymer | 334 | 37621.7 | 1 | UniProt (Q92905) Pfam (PF01398) Pfam (PF18323) | Homo sapiens (human) | |
| 2 | B (A) | COP9 signalosome complex subunit 1 | polymer | 527 | 59122.2 | 1 | UniProt (Q13098) Pfam (PF10602) Pfam (PF01399) Pfam (PF21151) | Homo sapiens (human) | SGN1,Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH |
| 3 | C (B) | COP9 signalosome complex subunit 2 | polymer | 443 | 51664.6 | 1 | UniProt (P61201) Pfam (PF01399) | Homo sapiens (human) | SGN2,Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TR-interacting protein 15,TRIP-15 |
| 4 | D (C) | COP9 signalosome complex subunit 3 | polymer | 423 | 47924.0 | 1 | UniProt (Q9UNS2) Pfam (PF22788) Pfam (PF01399) Pfam (PF21215) | Homo sapiens (human) | SGN3,Signalosome subunit 3,JAB1-containing signalosome subunit 3 |
| 5 | E (D) | COP9 signalosome complex subunit 4 | polymer | 406 | 46322.7 | 1 | UniProt (Q9BT78) Pfam (PF18420) Pfam (PF22241) Pfam (PF01399) | Homo sapiens (human) | SGN4,Signalosome subunit 4,JAB1-containing signalosome subunit 4 |
| 6 | F (F) | COP9 signalosome complex subunit 6 | polymer | 327 | 36203.4 | 1 | UniProt (Q7L5N1) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (human) | SGN6,Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP |
| 7 | G (G) | COP9 signalosome complex subunit 7b | polymer | 264 | 29656.9 | 1 | UniProt (Q9H9Q2) Pfam (PF22061) Pfam (PF01399) Pfam (PF18392) | Homo sapiens (human) | SGN7b,Signalosome subunit 7b,JAB1-containing signalosome subunit 7b |
| 8 | H (H) | COP9 signalosome complex subunit 8 | polymer | 209 | 23245.5 | 1 | UniProt (Q99627) Pfam (PF10075) | Homo sapiens (human) | SGN8,Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8 |
| 9 | I, K (I, M) | Kelch repeat and BTB domain-containing protein 2 | polymer | 623 | 71431.4 | 2 | UniProt (Q8IY47) Pfam (PF00651) Pfam (PF07707) Pfam (PF01344) | Homo sapiens (human) | BTB and kelch domain-containing protein 1 |
| 10 | J (L) | Cullin-3 | polymer | 768 | 89063.3 | 1 | UniProt (Q13618) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (human) | CUL-3 |
| 11 | L (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 12 | M, N, O, P (E, R) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A: 52 - 527 (UniProt: Q13098)
I, M: 1 - 623 (UniProt: Q8IY47)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Arg 2 | - | expression tag |
| Asp 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Ala 6 | - | expression tag |
| Pro 7 | - | expression tag |
| Ser 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Ala 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Val 14 | - | expression tag |
| Thr 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Leu 17 | - | expression tag |
| Tyr 18 | - | expression tag |
| Cys 19 | - | expression tag |
| Thr 20 | - | expression tag |
| Pro 21 | - | expression tag |
| His 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Arg 25 | - | expression tag |
| Ser 26 | - | expression tag |
| Asp 27 | - | expression tag |
| Leu 28 | - | expression tag |
| Val 29 | - | expression tag |
| Leu 30 | - | expression tag |
| Pro 31 | - | expression tag |
| Gly 32 | - | expression tag |
| Thr 33 | - | expression tag |
| Ala 34 | - | expression tag |
| Gly 35 | - | expression tag |
| Asp 36 | - | expression tag |
| Phe 37 | - | expression tag |
| Ser 38 | - | expression tag |
| Leu 39 | - | expression tag |
| Ser 40 | - | expression tag |
| Ala 41 | - | expression tag |
| Ser 42 | - | expression tag |
| Leu 43 | - | expression tag |
| Ser 44 | - | expression tag |
| Ala 45 | - | expression tag |
| Cys 46 | - | expression tag |
| Thr 47 | - | expression tag |
| Leu 48 | - | expression tag |
| Leu 49 | - | expression tag |
| Tyr 50 | - | expression tag |
| Glu 51 | - | expression tag |
| - | Arg 103 | deletion |
| - | Ser 104 | deletion |
| - | Ser 105 | deletion |
| - | Leu 106 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Asp 252 | Ser 252 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 575977.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 576238.7 |
