8GZG
Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 328 | 36657.3 | 2 | UniProt (P73297) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Synechocystis sp. PCC 6803 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1104 | 123719.8 | 1 | UniProt (P77965) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Synechocystis sp. PCC 6803 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit gamma | polymer | 626 | 71073.0 | 1 | UniProt (P74177) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) | Synechocystis sp. PCC 6803 | RNAP subunit gamma,RNA polymerase subunit gamma,Transcriptase subunit gamma |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 76 | 8749.8 | 1 | UniProt (P74352) Pfam (PF01192) | Synechocystis sp. PCC 6803 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor SigA | polymer | 429 | 50335.4 | 1 | UniProt (P74565) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Synechocystis sp. PCC 6803 | |
| 6 | G (Z) | DNA-directed RNA polymerase subunit beta' | polymer | 1323 | 145372.0 | 1 | UniProt (P73334) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Synechocystis sp. PCC 6803 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (1) | Nontemplate strand DNA | polymer | 67 | 20810.3 | 1 | synthetic construct | ||
| 8 | I (2) | Template strand DNA | polymer | 67 | 20673.2 | 1 | synthetic construct | ||
| 9 | J (3) | RNA (5'-R(*UP*CP*GP*A)-3') | polymer | 4 | 1240.8 | 1 | synthetic construct | ||
| 10 | K (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | L, M (D, Z) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 318 (UniProt: P73297)
C: 1 - 1102 (UniProt: P77965)
F: 1 - 425 (UniProt: P74565)
Z: 1 - 1317 (UniProt: P73334)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 515288.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 515444.0 |
