8GZG

Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	328	36657.3	2	UniProt (P73297) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Synechocystis sp. PCC 6803	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1104	123719.8	1	UniProt (P77965) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Synechocystis sp. PCC 6803	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit gamma	polymer	626	71073.0	1	UniProt (P74177) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) In PDB	Synechocystis sp. PCC 6803	RNAP subunit gamma,RNA polymerase subunit gamma,Transcriptase subunit gamma
4	E	DNA-directed RNA polymerase subunit omega	polymer	76	8749.8	1	UniProt (P74352) Pfam (PF01192) In PDB	Synechocystis sp. PCC 6803	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor SigA	polymer	429	50335.4	1	UniProt (P74565) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Synechocystis sp. PCC 6803
6	Z	DNA-directed RNA polymerase subunit beta'	polymer	1323	145372.0	1	UniProt (P73334) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Synechocystis sp. PCC 6803	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7	1	Nontemplate strand DNA	polymer	67	20810.3	1		synthetic construct
8	2	Template strand DNA	polymer	67	20673.2	1		synthetic construct
9	3	RNA (5'-R(*UP*CP*GP*A)-3')	polymer	4	1240.8	1		synthetic construct
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	D, Z	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 1 - 318 (UniProt: P73297)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

C: 1 - 1102 (UniProt: P77965)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

F: 1 - 425 (UniProt: P74565)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag

Z: 1 - 1317 (UniProt: P73334)

PDB	External Database	Details
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	515288.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	515444.0

*Water molecules are not included.