8GT2

Crystal structure of human cardiac alpha actin P109A mutant (AMPPNP state) in complex with fragmin F1 domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Actin, alpha cardiac muscle 1	polymer	391	43618.5	1	UniProt (P68032) Pfam (PF00022)	Homo sapiens (human)	Alpha-cardiac actin
2	B (B)	Actin-binding protein fragmin P	polymer	162	18253.4	1	UniProt (Q94707) Pfam (PF00626)	Physarum polycephalum
3	C (A)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F, K, L, M... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
6	G, H (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	I, J (B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	O, P (A, B)	water	water		18.0	711	Chemie (HOH)

Sequence modifications

A: 1 - 375 (UniProt: P68032)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Trp -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Pro -10	-	expression tag
Gln -9	-	expression tag
Phe -8	-	expression tag
Glu -7	-	expression tag
Lys -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ala 109	Pro 111	engineered mutation

B: 1 - 160 (UniProt: Q94707)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61872.0
Non-Polymers*	Number of molecules	12
	Total formula weight	1173.0
All*	Total formula weight	63045.0

*Water molecules are not included.