8GT1

Crystal structure of human cardiac alpha actin A108G mutant (ADP-Pi state) in complex with fragmin F1 domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Actin, alpha cardiac muscle 1	polymer	391	43630.5	1	UniProt (P68032) Pfam (PF00022)	Homo sapiens (human)	Alpha-cardiac actin
2	B (B)	Actin-binding protein fragmin P	polymer	162	18253.4	1	UniProt (Q94707) Pfam (PF00626)	Physarum polycephalum
3	C (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5	E, G, H (A)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
6	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	I, L, M (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
8	J, K (B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
9	N, O (A, B)	water	water		18.0	870	Chemie (HOH)

Sequence modifications

A: 1 - 375 (UniProt: P68032)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Trp -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Pro -10	-	expression tag
Gln -9	-	expression tag
Phe -8	-	expression tag
Glu -7	-	expression tag
Lys -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Gly 108	Ala 110	engineered mutation

B: 1 - 160 (UniProt: Q94707)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61884.0
Non-Polymers*	Number of molecules	11
	Total formula weight	1510.0
All*	Total formula weight	63393.9

*Water molecules are not included.