8GSW
Crystal structure of human cardiac alpha actin A108G mutant (AMPPNP state) in complex with fragmin F1 domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Actin, alpha cardiac muscle 1 | polymer | 391 | 43630.5 | 1 | UniProt (P68032) Pfam (PF00022) | Homo sapiens (human) | Alpha-cardiac actin |
| 2 | B (B) | Actin-binding protein fragmin P | polymer | 160 | 18099.3 | 1 | UniProt (Q94707) Pfam (PF00626) | Physarum polycephalum | |
| 3 | C (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 6 | F, G, H, K, L... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 7 | I, J (B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 8 | N, O (A, B) | water | water | 18.0 | 756 | Chemie (HOH) |
Sequence modifications
A: 1 - 375 (UniProt: P68032)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| Trp -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| Pro -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Ile -3 | - | expression tag |
| Glu -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Gly 108 | Ala 110 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 61729.8 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1078.0 | |
| All* | Total formula weight | 62807.8 |
