8GRU
Crystal structure of a constitutively active mutant of the alpha beta heterodimer of human IDH3 in complex with ICT, NAD and Ca
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Human IDH3 alpha subunit | polymer | 339 | 36625.1 | 2 | UniProt (P50213) Pfam (PF00180) In PDB | Homo sapiens (human) | |
2 | B, D | Isoform A of Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial | polymer | 352 | 39152.0 | 2 | UniProt (O43837-2) Pfam (PF00180) UniProt (by SIFTS) (O43837) In PDB | Homo sapiens (human) | Isocitric dehydrogenase subunit beta,NAD(+)-specific ICDH subunit beta |
3 | A, B, C, D | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 4 | Chemie (NAD) | |||
4 | A, C | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
5 | A, C | ISOCITRIC ACID | non-polymer | 192.1 | 2 | Chemie (ICT) |
Sequence modifications
A, C: 1 - 339 (UniProt: P50213)
B, D: 1 - 340 (UniProt: O43837-2)
PDB | External Database | Details |
---|---|---|
Ala 139 | Gln 166 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Glu 341 | - | expression tag |
Ile 342 | - | expression tag |
Cys 343 | - | expression tag |
Arg 344 | - | expression tag |
Arg 345 | - | expression tag |
Val 346 | - | expression tag |
Lys 347 | - | expression tag |
Asp 348 | - | expression tag |
Leu 349 | - | expression tag |
Asp 350 | - | expression tag |
Glu 351 | - | expression tag |
Asn 352 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 151554.3 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 3118.1 | |
All* | Total formula weight | 154672.4 |