8GQB
Crystal structure of lasso peptide epimerase MslH D11A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly-gamma-glutamate synthesis protein (Capsule biosynthesis protein) | polymer | 440 | 47275.4 | 1 | UniProt (A0A4V2TW40) | Streptomyces sp. | |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | D (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | E (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 6 | F (A) | water | water | 18.0 | 127 | Chemie (HOH) |
Sequence modifications
A: 1 - 440 (UniProt: A0A4V2TW40)
| PDB | External Database | Details |
|---|---|---|
| Ala 11 | Asp 11 | engineered mutation |
| Arg 34 | His 34 | conflict |
| Val 59 | Ala 59 | conflict |
| Met 101 | Leu 101 | conflict |
| His 287 | Asp 287 | conflict |
| Ala 380 | Val 380 | conflict |
| Ile 425 | Val 425 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 47275.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 360.4 | |
| All* | Total formula weight | 47635.8 |
