8GQ6

Cryo-EM Structure of the KBTBD2-CUL3-Rbx1 dimeric complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Kelch repeat and BTB domain-containing protein 2	polymer	623	71403.4	2	UniProt (Q8IY47) Pfam (PF00651) Pfam (PF07707) Pfam (PF01344)	Homo sapiens (human)
2	B, F (E, D)	E3 ubiquitin-protein ligase RBX1	polymer	121	13970.8	2	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
3	C, E (C, F)	Cullin-3	polymer	776	90105.5	2	UniProt (Q13618) Pfam (PF00888) Pfam (PF26557) Pfam (PF10557)	Homo sapiens (human)
4	G, H, I, J, K... (E, D)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

E, D: 1 - 108 (UniProt: P62877)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

C, F: 1 - 768 (UniProt: Q13618)

PDB	External Database	Details
Trp -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
Pro -4	-	expression tag
Gln -3	-	expression tag
Phe -2	-	expression tag
Glu -1	-	expression tag
Lys 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	350959.2
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	351351.6

*Water molecules are not included.