8GLX

CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 6:2:1 stoichiometry from E. coli Tn7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, F, G, I... (G, E, F, C, A...)	Transposon Tn7 transposition protein TnsC	polymer	523	59318.9	6	UniProt (P05846) Pfam (PF13401)	Escherichia coli	Protein E
2	B, H (X, Y)	Transposon Tn7 transposition protein TnsD	polymer	318	36636.0	2	UniProt (P13991) Pfam (PF06527) Pfam (PF15978)	Escherichia coli
3	C (H)	DNA (50-MER)	polymer	50	15438.9	1		Escherichia coli
4	D (I)	DNA (50-MER)	polymer	50	15366.8	1		Escherichia coli
5	K, R (G, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
6	L, O, Q, S, V... (G, E, F, C, A...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	M, T (X, Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8	N, P, U, W (E, F, A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

G, E, F, C, A, B: 1 - 503 (UniProt: P05846)

PDB	External Database	Details
Gly 2	Ser 2	engineered mutation
Pro 504	-	expression tag
Asn 505	-	expression tag
Ser 506	-	expression tag
Ser 507	-	expression tag
Ser 508	-	expression tag
Val 509	-	expression tag
Asp 510	-	expression tag
Lys 511	-	expression tag
Leu 512	-	expression tag
Ala 513	-	expression tag
Ala 514	-	expression tag
Ala 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	459991.3
Non-Polymers*	Number of molecules	14
	Total formula weight	2999.8
All*	Total formula weight	462991.2

*Water molecules are not included.