8GLW

CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 7:2:1 stoichiometry from E. coli Tn7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, E, F... (A, B, C, D, E...)	Transposon Tn7 transposition protein TnsC	polymer	523	59318.9	7	UniProt (P05846) Pfam (PF13401)	Escherichia coli	Protein E
2	D, I (Y, X)	Transposon Tn7 transposition protein TnsD	polymer	318	36636.0	2	UniProt (P13991) Pfam (PF06527) Pfam (PF15978)	Escherichia coli
3	J (H)	DNA (50-MER)	polymer	50	15438.9	1		Escherichia coli
4	K (I)	DNA (50-MER)	polymer	50	15366.8	1		Escherichia coli
5	L, N, S, U, X (A, B, D, E, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
6	M, O, Q, T, V... (A, B, C, D, E...)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
7	P, Y (C, G)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
8	AA, R (X, Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B, C, D, E, F, G: 1 - 503 (UniProt: P05846)

PDB	External Database	Details
Gly 2	Ser 2	engineered mutation
Pro 504	-	expression tag
Asn 505	-	expression tag
Ser 506	-	expression tag
Ser 507	-	expression tag
Ser 508	-	expression tag
Val 509	-	expression tag
Asp 510	-	expression tag
Lys 511	-	expression tag
Leu 512	-	expression tag
Ala 513	-	expression tag
Ala 514	-	expression tag
Ala 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	519310.3
Non-Polymers*	Number of molecules	16
	Total formula weight	3451.3
All*	Total formula weight	522761.6

*Water molecules are not included.