8GLU

CryoEM structure of TnsC(1-503) bound to TnsD(1-318) from E.coli Tn7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, F, E	Transposon Tn7 transposition protein TnsC	polymer	523	59318.9	3	UniProt (P05846) Pfam (PF13401) In PDB	Escherichia coli	Protein E
2	X	Transposon Tn7 transposition protein TnsD	polymer	318	36636.0	1	UniProt (P13991) Pfam (PF06527) Pfam (PF15978) In PDB	Escherichia coli
3	G, F	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
4	G, F, E	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	X	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	E	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

G, F, E: 1 - 503 (UniProt: P05846)

PDB	External Database	Details
Gly 2	Ser 2	engineered mutation
Pro 504	-	expression tag
Asn 505	-	expression tag
Ser 506	-	expression tag
Ser 507	-	expression tag
Ser 508	-	expression tag
Val 509	-	expression tag
Asp 510	-	expression tag
Lys 511	-	expression tag
Leu 512	-	expression tag
Ala 513	-	expression tag
Ala 514	-	expression tag
Ala 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	214592.8
Non-Polymers*	Number of molecules	7
	Total formula weight	1579.9
All*	Total formula weight	216172.7

*Water molecules are not included.