8GHF

cryo-EM structure of hSlo1 in plasma membrane vesicles

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Calcium-activated potassium channel subunit alpha-1	polymer	1090	122440.1	4	UniProt (Q12791) Pfam (PF00520) Pfam (PF22614) Pfam (PF03493) Pfam (PF21014)	Homo sapiens (human)	BK channel,BKCA alpha,Calcium-activated potassium channel,subfamily M subunit alpha-1,K(VCA)alpha,KCa1.1,Maxi K channel,MaxiK,Slo-alpha,Slo1,Slowpoke homolog,Slo homolog,hSlo
2	E, LA, TB, ZC (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	AD, BD, F, G, MA... (D, A, B, C)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
4	AA, AE, BB, BC, CB... (A, D, B, C)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	88	Chemie (POV)
5	AB, AC, BA, CA, CC... (B, C, A, D)	CHOLESTEROL	non-polymer		386.7	24	Chemie (CLR)
6	BE, GA, MB, VC (D, A, B, C)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)

Sequence modifications

A, B, C, D: 2 - 1056 (UniProt: Q12791)

PDB	External Database	Details
Met -15	-	expression tag
Ala -14	-	expression tag
Pro -13	-	expression tag
Ser -12	-	expression tag
Arg -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Glu -7	-	expression tag
Leu -6	-	expression tag
Arg -5	-	expression tag
Arg -4	-	expression tag
Arg -3	-	expression tag
Leu -2	-	expression tag
Thr -1	-	expression tag
Glu 0	-	expression tag
Pro 1	-	expression tag

A, B, C, D: 1401 - 1418 (PDB: 8GHF)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	489760.4
Non-Polymers*	Number of molecules	128
	Total formula weight	76676.2
All*	Total formula weight	566436.6

*Water molecules are not included.