8GDC

Cryo-EM Structure of the Prostaglandin E2 Receptor 3 Coupled to G Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein subunit gamma	polymer	108	12104.9	1	UniProt (A0A7J7XNR4) Pfam (PF00631) UniProt (by SIFTS) (P59768) In PDB	Homo sapiens (human)
4	R	Prostaglandin E2 receptor EP3 subtype	polymer	390	43357.7	1	UniProt (P43115) Pfam (PF00001) In PDB	Homo sapiens (human)	PGE receptor EP3 subtype,PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor
5	R	(Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid	non-polymer		352.5	1	Chemie (P2E)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C: -9 - 71 (UniProt: A0A7J7XNR4)

PDB	External Database	Details
Met -36	-	expression tag
Trp -35	-	expression tag
Arg -34	-	expression tag
Glu -33	-	expression tag
Leu -32	-	expression tag
Pro -31	-	expression tag
Leu -30	-	expression tag
Gly -29	-	expression tag
Leu -28	-	expression tag
Gly -27	-	expression tag
Glu -26	-	expression tag
Leu -25	-	expression tag
His -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
His -21	-	expression tag
Gln -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Arg -17	-	expression tag
Lys -16	-	expression tag
Leu -15	-	expression tag
Glu -14	-	expression tag
Pro -13	-	expression tag
Glu -12	-	expression tag
Leu -11	-	expression tag
Trp -10	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135325.8
Non-Polymers*	Number of molecules	1
	Total formula weight	352.5
All*	Total formula weight	135678.2

*Water molecules are not included.