8GDB
Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45727.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 344 | 37671.1 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein subunit gamma | polymer | 108 | 12104.9 | 1 | UniProt (A0A7J7XNR4) Pfam (PF00631) UniProt (by SIFTS) (P59768) | Homo sapiens (human) | |
| 4 | D (N) | NB35 | polymer | 128 | 13885.4 | 1 | Lama glama (llama) | ||
| 5 | E (R) | Prostaglandin E2 receptor EP4 subtype | polymer | 488 | 53173.3 | 1 | UniProt (P35408) Pfam (PF00001) | Homo sapiens (human) | PGE receptor EP4 subtype,PGE2 receptor EP4 subtype,Prostanoid EP4 receptor |
| 6 | F (R) | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid | non-polymer | 352.5 | 1 | Chemie (P2E) PubChem (16219826) PubChem (71195864) PubChem (176729059) PubChem (177546178) PubChem (35021823) PubChem (35021833) PubChem (26436117) PubChem (40575690) PubChem (35027359) PubChem (40575698) PubChem (44444717) PubChem (49822699) PubChem (91886115) PubChem (5280360) PubChem (97041839) PubChem (53477468) PubChem (54466054) PubChem (54466055) PubChem (54466056) PubChem (54466057) PubChem (57357999) PubChem (12314587) PubChem (156359195) PubChem (171042408) PubChem (59294054) PubChem (59881728) PubChem (59926133) PubChem (59941509) PubChem (59968515) PubChem (67937739) PubChem (70419675) PubChem (71082424) PubChem (71401211) PubChem (73357245) PubChem (90689772) PubChem (90798199) PubChem (91019309) PubChem (92450042) PubChem (92966520) PubChem (97041838) PubChem (158) PubChem (122176715) PubChem (9691) PubChem (122702174) PubChem (123841661) PubChem (122348) PubChem (124350425) PubChem (124406582) PubChem (124406583) PubChem (124524501) PubChem (3246816) PubChem (3246832) PubChem (5283061) PubChem (5283086) PubChem (5283116) PubChem (5283213) PubChem (5353587) PubChem (6445999) PubChem (6450019) PubChem (9874974) PubChem (9950404) PubChem (10247450) PubChem (11221902) PubChem (11581143) PubChem (12041746) PubChem (12314577) PubChem (12314580) PubChem (12314582) PubChem (12314584) PubChem (12314585) PubChem (12314588) PubChem (12314590) PubChem (12314594) PubChem (131954610) PubChem (139503973) PubChem (149202340) PubChem (154166399) PubChem (155114512) PubChem (156358885) PubChem (156359009) PubChem (156681952) PubChem (156681960) PubChem (162705518) PubChem (163091488) PubChem (163091489) PubChem (166639831) PubChem (166639839) PubChem (166639992) PubChem (169502219) PubChem (171157690) PubChem (172878425) PubChem (173974797) PubChem (175594575) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
G: -9 - 71 (UniProt: A0A7J7XNR4)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Asp 188 | Ala 188 | conflict |
| Ala 226 | Gly 226 | conflict |
| Ala 268 | Glu 268 | conflict |
| Lys 271 | Asn 271 | conflict |
| Asp 274 | Lys 274 | conflict |
| Lys 280 | Arg 280 | conflict |
| Asp 284 | Thr 284 | conflict |
| Thr 285 | Ile 285 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -36 | - | expression tag |
| Trp -35 | - | expression tag |
| Arg -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Leu -32 | - | expression tag |
| Pro -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Glu -26 | - | expression tag |
| Leu -25 | - | expression tag |
| His -24 | - | expression tag |
| Lys -23 | - | expression tag |
| Asp -22 | - | expression tag |
| His -21 | - | expression tag |
| Gln -20 | - | expression tag |
| Ala -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Leu -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Glu -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Trp -10 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 162562.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 352.5 | |
| All* | Total formula weight | 162914.7 |
