8GDA
Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45727.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38458.0 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | |
| 3 | C (G) | Guanine nucleotide-binding protein subunit gamma | polymer | 108 | 12104.9 | 1 | UniProt (A0A7J7XNR4) Pfam (PF00631) UniProt (by SIFTS) (P59768) | Homo sapiens (human) | |
| 4 | D (N) | NB35 | polymer | 128 | 13885.4 | 1 | Lama glama (llama) | ||
| 5 | E (R) | Prostaglandin E2 receptor EP4 subtype | polymer | 488 | 53173.3 | 1 | UniProt (P35408) Pfam (PF00001) | Homo sapiens (human) | PGE receptor EP4 subtype,PGE2 receptor EP4 subtype,Prostanoid EP4 receptor |
| 6 | F (R) | (2S,5R)-5-[(1E,3S)-4,4-difluoro-3-hydroxy-4-phenylbut-1-en-1-yl]-1-[6-(1H-tetrazol-5-yl)hexyl]pyrrolidin-2-ol | non-polymer | 421.5 | 1 | Chemie (YZV) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
G: -9 - 71 (UniProt: A0A7J7XNR4)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Asp 188 | Ala 188 | conflict |
| Ala 226 | Gly 226 | conflict |
| Ala 268 | Glu 268 | conflict |
| Lys 271 | Asn 271 | conflict |
| Asp 274 | Lys 274 | conflict |
| Lys 280 | Arg 280 | conflict |
| Asp 284 | Thr 284 | conflict |
| Thr 285 | Ile 285 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -36 | - | expression tag |
| Trp -35 | - | expression tag |
| Arg -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Leu -32 | - | expression tag |
| Pro -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Glu -26 | - | expression tag |
| Leu -25 | - | expression tag |
| His -24 | - | expression tag |
| Lys -23 | - | expression tag |
| Asp -22 | - | expression tag |
| His -21 | - | expression tag |
| Gln -20 | - | expression tag |
| Ala -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Leu -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Glu -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Trp -10 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 163349.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 421.5 | |
| All* | Total formula weight | 163770.6 |
