8GD9

Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	29145.1	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein subunit gamma	polymer	108	12104.9	1	UniProt (A0A7J7XNR4) Pfam (PF00631) UniProt (by SIFTS) (P59768) In PDB	Homo sapiens (human)
4	N	NB35	polymer	128	13885.4	1		Lama glama (llama)
5	R	Prostaglandin E2 receptor EP4 subtype	polymer	488	53173.3	1	UniProt (P35408) Pfam (PF00001) In PDB	Homo sapiens (human)	PGE receptor EP4 subtype,PGE2 receptor EP4 subtype,Prostanoid EP4 receptor
6	R	4-({2-[(1R,2R,3R,5S)-3,5-dihydroxy-2-{(3S)-3-hydroxy-4-[3-(methoxymethyl)phenyl]butyl}cyclopentyl]ethyl}sulfanyl)butanoic acid	non-polymer		440.6	1	Chemie (Z2C)

Sequence modifications

A: 1 - 197 (UniProt: P63092)
A: 198 - 394 (UniProt: P63092)

PDB	External Database	Details
Ala 226	Gly 227	conflict
Ala 268	Glu 269	conflict
Lys 271	Asn 272	conflict
Asp 274	Lys 275	conflict
Lys 280	Arg 281	conflict
Asp 284	Thr 285	conflict
Thr 285	Ile 286	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

G: -9 - 71 (UniProt: A0A7J7XNR4)

PDB	External Database	Details
Met -36	-	expression tag
Trp -35	-	expression tag
Arg -34	-	expression tag
Glu -33	-	expression tag
Leu -32	-	expression tag
Pro -31	-	expression tag
Leu -30	-	expression tag
Gly -29	-	expression tag
Leu -28	-	expression tag
Gly -27	-	expression tag
Glu -26	-	expression tag
Leu -25	-	expression tag
His -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
His -21	-	expression tag
Gln -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Arg -17	-	expression tag
Lys -16	-	expression tag
Leu -15	-	expression tag
Glu -14	-	expression tag
Pro -13	-	expression tag
Glu -12	-	expression tag
Leu -11	-	expression tag
Trp -10	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	147726.9
Non-Polymers*	Number of molecules	1
	Total formula weight	440.6
All*	Total formula weight	148167.5

*Water molecules are not included.