8GCP
Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein subunit gamma | polymer | 108 | 12104.9 | 1 | UniProt (A0A7J7XNR4) Pfam (PF00631) UniProt (by SIFTS) (P59768) | Homo sapiens (human) | |
| 4 | D (E) | scFv | polymer | 307 | 32898.8 | 1 | Homo sapiens (Human) | ||
| 5 | E (R) | Prostaglandin E2 receptor EP4 subtype | polymer | 488 | 53173.3 | 1 | UniProt (P35408) Pfam (PF00001) | Homo sapiens (human) | PGE receptor EP4 subtype,PGE2 receptor EP4 subtype,Prostanoid EP4 receptor |
| 6 | F (R) | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid | non-polymer | 352.5 | 1 | Chemie (P2E) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
C: -9 - 71 (UniProt: A0A7J7XNR4)
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 203 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -36 | - | initiating methionine |
| Trp -35 | - | expression tag |
| Arg -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Leu -32 | - | expression tag |
| Pro -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Glu -26 | - | expression tag |
| Leu -25 | - | expression tag |
| His -24 | - | expression tag |
| Lys -23 | - | expression tag |
| Asp -22 | - | expression tag |
| His -21 | - | expression tag |
| Gln -20 | - | expression tag |
| Ala -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Leu -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Glu -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Trp -10 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 178040.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 352.5 | |
| All* | Total formula weight | 178392.6 |
