8GAS

vFP48.02 Fab in complex with BG505 DS-SOSIP Env trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	L, A, F	vFP48.02 light chain	polymer	112	12270.9	3		Mus musculus (mouse)
2	H, C, I	vFP48.02 heavy chain	polymer	122	13383.9	3		Mus musculus (mouse)
3	G, D, J	Envelope glycoprotein gp120	polymer	481	54086.3	3	UniProt (Q2N0S6) Pfam (PF00516) In PDB	Human immunodeficiency virus 1 (HIV-1)
4	B, E, K	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S6) Pfam (PF00517) In PDB	Human immunodeficiency virus 1 (HIV-1)
5	a, e, f, M, Q...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	9	In PDB GlyTouCan (G15407YE)
6	b, c, d, N, O...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
7	g, S, Z	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
8	D, E, J, G, K...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	36	Chemie (NAG)

Sequence modifications

G, D, J: 31 - 508 (UniProt: Q2N0S6)

PDB	External Database	Details
Cys 201	Ile 200	conflict
Asn 332	Thr 330	conflict
Cys 433	Ala 430	conflict
Cys 501	Ala 498	conflict
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

B, E, K: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	290662.6
Branched	Number of molecules	21
	Total formula weight	11344.5
Non-Polymers*	Number of molecules	36
	Total formula weight	7963.5
All*	Total formula weight	309970.7

*Water molecules are not included.