8GAF

Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Cas7	polymer	283	32208.1	7	UniProt (A0A378VEU0) Pfam (PF05107) UniProt (by SIFTS) (D0W8X6) In PDB	Neisseria lactamica
2	G, H	Cas8	polymer	582	64715.7	2	UniProt (A0A1V0DVX6) Pfam (PF09709) UniProt (by SIFTS) (D0W8X5) In PDB	Neisseria lactamica
3	I, J	Cas11	polymer	124	14245.2	2	UniProt (A0A378VF47) UniProt (by SIFTS) (D0W8X5) In PDB	Neisseria lactamica
4	K	crRNA (43-MER)	polymer	43	13816.2	1		Neisseria lactamica
5	N	Cas5	polymer	205	23870.5	1	UniProt (D0W8X4) Pfam (PF09704) In PDB	Neisseria lactamica

Sequence modifications

A, B, C, D, E, F, M: 2 - 283 (UniProt: A0A378VEU0)

PDB	External Database	Details
Gly 284	-	expression tag

G, H: 1 - 582 (UniProt: A0A1V0DVX6)

PDB	External Database	Details
Ala 190	Val 190	conflict
Ala 239	Ile 239	conflict
Ile 242	Val 242	conflict
Gly 260	Ser 260	conflict
Thr 271	Ala 271	conflict
Ala 299	Glu 299	conflict
Ala 306	Thr 306	conflict
Cys 317	Gln 317	conflict
Glu 322	Lys 322	conflict
Asp 323	Glu 323	conflict
Ile 481	Thr 481	conflict
Tyr 562	Cys 562	conflict

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	421065.1
All*	Total formula weight	421065.1

*Water molecules are not included.